Quantifying mRNA levels across tissue sections with 2D-RT-qPCR
نویسندگان
چکیده
منابع مشابه
Quantifying mRNA levels across tissue sections with 2D-RT-qPCR.
Measurement of mRNA levels across tissue samples facilitates an understanding of how genes function and what their roles are in disease. Quantifying low-abundance mRNA requires a workflow that preserves spatial information, isolates RNA, and performs reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR). This is complex because these steps are typically performed in three separ...
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BACKGROUND The spatiotemporal expression patterns of microRNAs (miRNAs) are important to the verification of their predicted function. RT-qPCR is the accepted technique for the quantification of miRNA expression; however, stem-loop RT-PCR and poly(T)-adapter assay, the two most frequently used methods, are not very convenient in practice and have poor specificity, respectively. RESULTS We hav...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2011
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-011-5062-8